Nano-manufacturing processes for reconstruction of tissue barriers and generation of uniform organoids
Stem cell technology has emerged as a powerful tool on the way to ultimate human health and it has been utilized in various biomedical fields including regenerative medicine, tissue engineering, and in vitro physiological model systems. The key of stem cell technology is guiding the stem cells to have in vivo physiological cellular phenotype, functions, and behaviors. Among the various approaches to guide the stem cell fate and behaviors, providing the in vivo-like microenvironments to the stem cells based on the nano-/micro manufacturing processes has been highlighted as a promising method for the last decades. Though the nano-/micro- manufacturing processes have been greatly contributed to developing functional and biomimetic culture scaffolds, however, it is still difficult but desired to develop an advanced culture scaffold possessing in vivo-like complex microenvironments including biophysical, biochemical, and/or structural cues. We suggest several nano-manufacturing solutions to develop microphysiological scaffolds providing in vivo-like microenvironments to the stem cells by elaborately utilizing nanofibrous membranes. The...